WebOct 21, 2024 · Complex cell-based assays that aim to mimic the in vivo cellular environment are playing an increasing role in drug discovery and development. Whilst, the specific details of such assays are beyond the scope of this article, it is important to emphasize that the basic principles of assay development must be adhered to in order to … WebSep 14, 2016 · Cell-based assays have been a key component in streamlining the drug development process, helping drugs be brought to the market in a quick and efficient manner, and their technological evolution …
Biology Cell-Based Assays Charles River
WebFor example, a protein product might have a functional domain that allows for the product to be internalized into the cell, a domain that allows it to move to the nucleus and another … WebMar 1, 2010 · Cell-based assays for ErbB-2 receptor lead optimization. Compounds were tested for the inhibition of ErbB-2 kinase activity [ 55 ], cellular proliferation [ 12] and cellular autophosphosphorylation (cellular PY) [ 14 ]. Inhibition potency was expressed as the negative log (pIC 50) of the concentration that produced 50% inhibition (IC 50 ). draw something fun at home answers
Choosing the right cell-based assay for your research
WebAgain, if we take the example of a functional assay designed to assess the endocytosis of a ligand into a cell, the protein under test could be tagged with GFP and the ligand, such as transferrin, could be labelled with a red fluorophore such as rhodamine. ... Such cell‐based functional assays can therefore be envisaged for any cellular ... WebDetecting and quantifying cellular processes such as cell death or metabolic reactions requires high quality tools. We have over 750 cellular and biochemical assays, covering … WebAug 27, 2024 · In subsequent cell-based BST-2 degradation assays, inhibitor Y-39983 HCl restored the cell-surface and total cellular level of BST-2 in the presence of Vpu. ... For example, human immunodeficiency virus (HIV)-1 encodes a specific accessory protein, viral protein U (Vpu), to counteract BST-2 by binding to it and directing its lysosomal ... draw something game download