2024 Rpl22ha

Rpl22ha

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August undefined, 2024

Web(b) Meta-gene analysis of LTM-associated ribosome density (top panel) or CHX-associated ribosome density (bottom panel) in MEF cells (blue line) and liver cells (red line). …

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WebOverall design All DRG neurons from young adult (3-6 mo) male mice expressing Rpl22HA/HA Runx3-Cre+ and age/sex matched Cre negative littermates (Rpl22HA/HA Runx3-Cre-) were harvested using density purification on Percoll and grown for 24h in serum-supplemented F12 medium. WebSpecifically, we used Cx 3 cr1 creER mice 26 in combination with a Rpl22 HA RiboTag allele 27 , in which tamoxifen (TAM)-induced Cre recombinase activation leads to permanent expression of... optica bassi

011029 - RiboTag Strain Details - The Jackson Laboratory

WebRibosomal protein L22 Alt name Rpl22 Species M. musculus (mouse) Insert Size (bp) 489 GenBank ID NM_001277113.1 Entrez Gene Rpl22 ( a.k.a. 2700038K18Rik) Tag / Fusion … WebBigenic male and female progeny were injected with tamoxifen at 6 weeks of age to induce the expression of Rpl22HA (RiboTag) specifically in astrocytes (Figures S2A–S2E). Three weeks later, the mice were injected with LPS (or PBS) to trigger neuroinflammation. WebApr 24, 2024 · Using mice expressing an exogenous HA tag on ribosomes (Rpl22HA) specifically in astrocytes ( Aldh1l1 -cre/ERT2 x Ribotag mice) we sequenced polysomic … portillo\u0027s investor relations

017911 - Esr1-Cre Strain Details - The Jackson Laboratory

Nkx2 1 Cre Recombinase Cdna Forward Roche Bioz

Web2009. Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Sanz E , et al. PubMed: 19666516. MGI: J:151962. Proc Natl Acad Sci U S A 106 (33):13939-44. … WebDec 21, 2016 · Finally, we crossed the Aldh1l1-Cre/ERT2 mice with RiboTag mice that express the ribosomal protein Rpl22HA in a Cre-dependent manner (Sanz et al., 2009). We used this strategy to determine the cortical astrocyte transcriptome in adult mice, which we compared to data from postnatal day 7 (P7) mice (Zhang et al., 2014 ). optica bangaloreWebIL-1-induced enteric gliosis resembles the EGC reactivity profile after surgical trauma a Schematic overview of the RiboTag method applied in primary EGCs treated with IL-1β (10 ng/ml) or vehicle... optica benito

"WebC, Heatmap and relative log2 fold change of significantly altered translated transcripts in the chemokine pathway from neurons (Syn1Cre+/−Rpl22HA/+) and astrocytes (GFAPCre+/−Rpl22HA/+) in the OB of infected mice (each column represents one individual mouse, adjusted p 0.05). D, Infected mice were perfused at 6 dpi and brains were … " - Rpl22ha

Rpl22ha

Ribotag analysis reveals alternative specificities of Cx3cr1CreER …

WebDec 17, 2024 · The transcriptome of enteric glial cells (EGCs) was investigated using PLPCreERT2 Rpl22HA mice. In addition, we assessed the effect of supernatant from neurosphere-derived EGCs on monocyte differentiation based on the expression of pro- and anti-inflammatory factors. Results: Muscularis inflammation induced marked alterations … WebThe Rpl22-HA gene can also be introduced into the animal by stereotaxic injection of an AAV-DIO-Rpl22-HA that is then activated in Cre-expressing cells. Both methods for …

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WebJan 7, 2024 · Crossing RiboTag mice (RPL22 HA/WT) with specific Cre-driver mice: Cre-lox recombination approach is commonly used as a tool for cell-type-specific gene expression. The RiboTag mice express HA-tagged ribosome protein RPL22 in Cre-expressing cells. WebThis protocol describes an optimized method for RiboTag Immunoprecipitation from mouse brain tissue.

WebScale bars, 200 µm. e, Immunofluorescence staining of tissue sections of Cx3cr1Cre:Rpl22HA (left) and TAM-treated Cx3cr1CreER:Rpl22HA (right) mice, stained for IBA1, HA and Hoechst, showing ... WebRibosomal protein L22 ( Rpl22 Mouse) Use Tags HA Expression Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pVP91A-pcaHG Plasmid #113766 Purpose Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida. Depositor John Lipscomb …

WebMar 26, 2012 · Rpl22 HA 蛋白质主要存在于多聚核糖体片断中，在第1个梯度（细胞质）中几乎不存在。当加入EDTA后，导致核糖体总蛋白和Rpl22 HA 蛋白质变成更轻的片段，都 … WebNational Center for Biotechnology Information

WebSep 1, 2024 · In these mice, the HA-tagged ribosomal subunit Rpl22HA [37] was expressed selectively in astrocytes [38]. Differential expression analysis revealed that 1180 genes were significantly enriched in striatal astrocytes relative to the hippocampus; that is, astrocytes were molecularly separable in the striatum relative to the hippocampus, a ...

WebTissues obtained from the animals indicates in (B, C) are homogenized and the MGE-expressed, Rpl22-HA-associated mRNA are immunoprecipitated and sequenced in parallel with the bulk mRNA. Techniques Used: Expressing, Negative Control, Agarose Gel Electrophoresis, Amplification, Immunoprecipitation portillo\u0027s in bolingbrook ilWebR&D Systems polyclonal goat antimouse cxcl10 antibodies. Polyclonal Goat Antimouse Cxcl10 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars … optica bonicheWebSep 28, 2024 · (D) Immunoprecipitation of the HA-tagged ribosomal protein RPL22HA, which is a component of actively translating polyribosomes. The expression of HA-tagged … optica bergaWebThis strongest engine has a peak torque of 163 lb-ft of torque that is achieved at 6500 RPM. This is a respectable amount of power for a 2.157 liter, or 131.6 cubic inch, engine that is … portillo\u0027s in californiaWebWe used standard methods ( Figure 7 C,D) to immunoprecipitate (IP) Rpl22HA containing ribosomes and their associated actively translated mRNAs ( Sanz et al., 2009), and … optica blessWebGrowth-associated protein 43 (GAP43) is one compound used to indicate growth of axonal endings during development and regeneration, particularly of peripheral neurons. Using immunohistochemistry, we have studied the expression of GAP43 in the spinal cord of rats subjected to mild, moderate or severe … portillo\u0027s in crystal lake ilWeb2 days ago · Abstract. Astrocytes and neurons extensively interact in the brain. Identifying astrocyte and neuron proteomes is essential for elucidating the protein networks that dictate their respective ... portillo\u0027s ingredients