T4 uvsy recombinase
WebProduct Description. The T4 UvsX Recombinase derived from T4 Phage is the homologue of RecA/Rad51 family. It plays an important role in the repair of double-stranded DNA breaks …
T4 uvsy recombinase
Did you know?
WebT4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound … WebIntact Genomics (ig ®) Stable 2 electrocompetent E. coli cells offer the highest transformation efficiencies of ≥5 x 10 10 cfu/µg plasmid DNA which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. Stable 2 cells are capable of cloning methylated genomic sequences ...
WebJun 13, 2006 · The recombinase we have employed, T4 uvsX, binds cooperatively to oligonucleotides in the presence of ATP. The resulting nucleoprotein complex actively … WebNov 6, 1998 · The UvsX gene product of bacteriophage T4 is a 40 kDa protein which catalyzes general genetic recombination. The enzymatic functions of the UvsX recombinase are similar to those of the well-characterized RecA protein of Escherichia coli, including single-stranded (ss) DNA-dependent ATPase and strand-transferase activities.
WebBacteriophage T4 homologous recombination events are promoted by presynaptic filaments of UvsX recombinase bound to single-stranded DNA (ssDNA). UvsY, the phage … WebNov 6, 1998 · The UvsX gene product of bacteriophage T4 is a 40 kDa protein which catalyzes general genetic recombination. The enzymatic functions of the UvsX …
WebApr 11, 2024 · UvsX protein is the recombinant enzyme encoded by bacteriophage T4 and belongs to the RecA recombinase family [14,15,16]. ... Y. Function of cloned T4 recombination genes, uvsX and uvsY, in cells of Escherichia coli. Mol. Gen. Genet. 1988, 211, 350–356. [Google Scholar]
WebT4 UvsX, UvsY, and a single stranded binding protein T4 gp32 form D-loop recombination structures that initiate amplification by a strand-displacing DNA polymerase. RPA is … greenlancer solarWebAug 27, 2024 · Recombinase polymerase amplification (RPA) is an isothermal reaction that amplifies a target DNA sequence with a recombinase, a single-stranded DNA-binding protein (SSB), and a strand-displacing DNA polymerase. In this study, we optimized the reaction conditions of RPA to detect SARS-CoV-2 DNA and R … flyff axtWebDec 3, 2010 · UvsY is the only member of the core T4 recombination machinery that forms a discreet oligomeric structure: It exists as a stable hexamer of identical 15.8 kDa subunits in solution, and binds to ssDNA in this form [ 50 ]. UvsY binds to both ssDNA and dsDNA, but has a much higher affinity for the former under relaxed DNA conditions [ 51 ]. flyff ayerWebMar 7, 2016 · Structure and mechanism of the phage T4 recombination mediator protein UvsY Full Record Related Research Abstract The UvsY recombination mediator protein is critical for efficient homologous recombination in bacteriophage T4 and is the functional analog of the eukaryotic Rad52 protein. greenland 10000 years agoWebApr 5, 2010 · The T4 recombination mediator protein, UvsY, has emerged as a central player in this system, along with UvsX recombinase and Gp32, the T4 SSB protein. In vitro , UvsY stimulates the enzymatic activities of UvsX recombinase by promoting UvsX–ssDNA filament assembly, and by helping UvsX to displace bound Gp32 from ssDNA ( 10 , 13 ). flyff axesWebRPA, is it a good fit for your re... flyff azriaWebT4 UvsX DNA Recombinase; T4 UvsY Protein; T4 gp32 Protein; Bsu DNA Polymerase Large Fragment; Sau DNA Polymerase Large Fragment; Cloning Kits and Related Enzymes. Quick10™ Cloning Kit; ig-Fusion™ Cloning Kit; ig-Fusion™ Cloning Enzyme Premix; T4 DNA Ligase; Taq DNA Ligase; greenland 1909 expedition