The gpx4 activity assay kit
WebThe concentration of Glutathione Peroxidase 4 (GPX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve. ... MeSH code E05 478 566 350 … Web29 May 2024 · To detect the intracellular ROS levels in SW480 and HCT116 cells, Reactive Oxygen Species Assay Kit (S0033, Beyotime, China) was used. ... GPX4 activity assay. g …
The gpx4 activity assay kit
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WebThe Iron Assay kit (ab83366) was used for detection of iron content according to the instructions of manufacturer. To investigate the degree of iron deposit in the pancreas, Perls staining assay was performed. ... of GPX4 were decreased in diabetes, which were further increased by CCA, confirming the antioxidant effects of CCA. The GPX4 ... Web13 Sep 2024 · The activity of GPX4 was determined using a HT Glutathione Peroxidase Assay Kit (Trevigen, Inc. Gaithersburg, MD, USA). The assay principle was based on the …
WebHuman Glutathione Peroxidase 4 (GPX4) ELISA Kit: Product Code: HUDL01248: Size: 96 Assays: Target: GPX4: Synonyms: MCSP, PHGPx, SnGPx, SnPHGPx, Phospholipid … Web13 Apr 2024 · Cell viability was measured by trypan blue staining according to kit instruction. Measurements of MDA and SOD. Malondialdehyde (MDA) and superoxide dismutase (SOD) are indicators of lipid superoxide. MDA and SOD in H9c2 cell were measured using commercially available assay kits according to the manufacturer’s instruction and …
http://btyxyxb.btmc.edu.cn/CN/10.16833/j.cnki.jbmc.2024.04.001 Web15 Dec 2024 · The concentration of protein in the lysate was determined using BCA assay kit (Thermo Fisher, Cat# 23225) using BSA as standards. Then, on a 96-well plate, ... For …
WebCayman's GPX4 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human GPX4, a negative regulator of the ferroptosis …
WebProperties. Data. Description. Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis. Chemical Name. [4- [Bis (4-chlorophenyl)methyl]-1-piperazinyl] (5-methyl-4-nitro-3-isoxazolyl)methanone. Biological Activity. Glutathione peroxidase (GPX4) inhibitor. Kills mutant RAS-expressing cell lines (IC50 ... permanently crossword puzzle clue answerWeb10 Apr 2024 · Malondialdehyde (MDA) Assay Kit (competitive ELISA) (#ab238537, Abcam) was used for the rapid detection and quantitation of MDA-protein adducts. And lipid ROS … permanently crosswordWebThis immunoassay kit allows for the in vitro quantitative determination of Human GPX4 concentrations in serum plasma and other biological fluids. Sensitivity: Contact Technical … permanently connected deviceWeb1 May 2024 · GPx4 activity is central to the termination of cellular lipid peroxidation. The donor antioxidants tocopherol and ascorbate (TOH and AscH−) cooperatively remove … permanently cold handsWebProduct Details GPX4 ELISA Kit (hide) For the quantitative determination of mouse phospholipid hydroperoxide glutathione peroxidase, mitochondrial (GPX4) concentrations … permanently defineWeb31 Jan 2024 · Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 4 (GPX4) were tested 20 times on one plate, … permanently cold pillowWeb3 Oct 2024 · evaluate the effects of compound on GPX4 activity, compound was first pre-incubated with purified GPX4 in the assay buffer [Tris-HCl (100 mM, pH 7.4), EDT A (5 … permanently def