WebTosoh Bioscience LLC, a provider of chromatographic resins for the purification of biopharmaceutical drugs in commercial manufacturing processes, has announced the introduction of TOYOPEARL AF-rProtein L-650F, a Protein L resin that exhibits the highest binding capacity for antibody fragments that lack an Fc-portion (e.g. Fab, ScF Dab, etc.). WebMar 6, 2024 · TOYOPEARL AF-rProtein L-650F resin is now available for sale in the Americas from Tosoh Bioscience LLC. TOYOPEARL AF-rProtein L-650F resin offers a high binding capacity, but also has the ability to bind to antibody classes that do not have the affinity to Protein A, such as IgM and IgA.
TOYOPEARL® AF-rProtein A HC-650F: Economic Advantages of …
WebAug 10, 2016 · TOYOPEARL AF-rProtein A HC-650F and two other commercially available protein A resins (one of them also a “high capacity” resin) were compared on a cost-per-gram of monoclonal antibody (mAb) produced basis at multiple resin prices from $7,000 to $17,000 per liter as well as three different column configurations. For in-depth … WebAF-rProtein L-650F resin, 0.83 mL column volume with 10% breakthrough at 3.4 minutes and 4.0 minutes residence times to determine the dynamic binding capacity (DBC) of a Fab frag-ment on the resin. Table 1 shows the comparison data between TOYOPEARL AF-rProtein L-650F resin to its competitor 85 μm prowill wood floors
Tosoh Bioscience Introduces TOYOPEARL® AF-rProtein L-650F Process
TOYOPEARL AF-r Protein L-650F exhibits dynamic binding capacities (DBCs) of greater than 38 g/L for Fabs, almost twice the capacity of other commercial Protein L resins. Robust : The recombinant ligand has been engineered to maintain capacity even after repeated exposure to mild alkaline solution. WebTOYOPEARL AF-rProtein A HC-650F Chromatographic Conditions (1) Regular IgG Binding and intermediate washing •0.02 mol/L sodium phosphate buffer (pH 7.2), 0.15 mol/L NaCl •When other impurities like green pigment is adsorbed, additional stepwise washing with buffer at pH above 4 may be effective. WebAF-rProtein A HC-650F, AF-rProtein L-650F, NH2-750F, Sulfate-650F, GigaCap Q-650M, GigaCap S-650S 1 mL x 1 ea 0045232 SkillPak Best in Class 1 mL col. library 7 mm ID x … restaurants near town hall seattle